Smad4(DPC4) Antibody ( DCS46)

Basic information

Name

Smad4(DPC4) Antibody ( DCS46)

size

0,1 mg

Catalog number

MA1089

Price

320 €

Details & buy    

Extended information

Raised in

mice

Gene Name

SMAD4

Clone

DCS-46

Analyses

WB,ICC

Purification

Ascites

French translation

anticorps

Target antigen

Smad4DPC4

Product form

freeze-dried

Reacts with species:

Human, rabbit, rat

Clonality

Monoclonal antibody

Gene Full Name

SMAD family member 4

Type of the antibody

IgG1 monoclonal antibody

Immunogen

Recombinant human Smad4(DPC4).

Entrez GeneID

Human(4089), Mouse(17128), Rat(50554)

Protein Name

Mothers against decapentaplegic homolog 4

Uniprot ID

Human(Q13485), Mouse(P97471), Rat(O70437)

Product configuration

Mouse ascites fluid, 1.2% Na acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Solubilization

The powdered antibody should be dissolved in 1ml of PBS buffer to achieve final concentration of 100ug/ml.

Properties

If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Tips

The Smad4(DPC4) Antibody ( DCS46) did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.

Synonyms

JIP|DPC4|MADH4|MYHRS|MAD homolog 4|MAD,mothers against decapentaplegic homolog 4(Drosophila)|SMAD, mothers against DPP homolog 4(Drosophila)|SMA- AND MAD-RELATED PROTEIN 4|DELETED IN PANCREATIC CARCINOMA 4|Deletion target in pancreatic carcinoma 4|SMAD family member 4

Storage condtions

Keep the Smad4(DPC4) Antibody ( DCS46)at minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.

Background

SMAD4 plays a pivotal role in signal transduction of the transforming growth factor beta superfamily cytokines by mediating transcriptional activation of target genes. Smad4 signalling in T cells is required for suppression of gastrointestinal cancer. Mutational inactivation of SMAD4 causes TGF-beta unresponsiveness and gave a basis for understanding the physiologic role of this gene in tumorigenesis. Mutations in DPC4(SMAD4) cause juvenile polyposis syndrome, but only account for a minority of cases.

Related articles

1. Kim, B.-G.; Li, C.; Qiao, W.; Mamura, M.; Kasprzak, B.; Anver, M.; Wolfraim, L.; Hong, S.; Mushinski, E.; Potter, M.; Kim, S.-J.; Fu, X.-Y.; Deng, C.; Letterio, J. J. : Smad4 signalling in T cells is required for suppression of gastrointestinal cancer. Nature 441: 1015-1019, 2006. Note: Erratum: Nature 444: 780 only, 2006. 2. Houlston, R.; Bevan, S.; Williams, A.; Young, J.; Dunlop, M.; Rozen, P.; Eng, C.; Markie, D.; Woodford-Richens, K.; Rodriguez-Bigas, M. A.; Leggett, B.; Neale, K.; Phillips, R.; Sheridan, E.; Hodgson, S.; Iwama, T.; Eccles, D.; Bodmer, W.; Tomlinson, I. : Mutations in DPC4 (SMAD4) cause juvenile polyposis syndrome, but only account for a minority of cases. Hum. Molec. Genet. 7: 1907-1912, 1998. 3. Howe, J. R.; Roth, S.; Ringold, J. C.; Summers, R. W.; Jarvinen, H. J.; Sistonen, P.; Tomlinson, I. P. M.; Houlston, R. S.; Bevan, S.; Mitros, F. A.; Stone, E. M.; Aaltonen, L. A. : Mutations in the SMAD4/DPC4 gene in juvenile polyposis. Science 280: 1086-1088, 1998.