Anti-Smad1/5 (Ser463 + Ser465), ALEXA Fluor 594

Basic information


Anti-Smad1/5 (Ser463 + Ser465), ALEXA Fluor 594


100 microliters

Catalog number



516 €

Extended information

Swiss Prot




Gene ID

4086, 4090


1ug per 1ul

Excitation emission




Modification site

Ser463 + Ser465


Alexa conjugate 1

Conjugated with


Subcellular locations

Cytoplasm, Nucleus

Applications with corresponding dilutions



Polyclonal Antibody


Polyclonal Antibodies

Purification method

Purified by Protein A.

Target Protein/Peptide

Smad1/5 Ser463 + Ser465


Conjugated Primary Antibody


Alexa Fluor,ALEXA FLUOR® 594

Other name

Anti-Smad1/5 (Ser463 + Ser465)

Host organism

Rabbit (Oryctolagus cuniculus)

Also known as

Smad1/5 (Ser463 + Ser465) Antibody


For facs or microscopy Alexa 1 conjugate.

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Antigen Source

KLH conjugated synthetic phosphopeptide derived from human Smad1/5 around the phosphorylation site of Ser463/465


Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.


This phosphorylation site is homologous in the listed cross reactive species at the specified location.Smad1/5 (Ser463 + Ser465)


This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.


Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.


Smad1 phospho S463/465; Smad1 phospho Ser463/465; p-Smad1 Ser463/465; Mothers against decapentaplegic homolog 1; BSP 1; BSP1; BSP-1; Dwarfin A; DwfA; hSMAD 1; hSMAD1; JV 41; JV4 1; JV4-1; JV41; MAD mothers against decapentaplegic homolog 1; Mad related protein 1; MADH 1; MADH1; Madr 1; MADR1; Mothers against decapentaplegic homolog 1; Mothers against DPP homolog 1; Sma and Mad related protein 1; SMAD 1; SMAD-1; SMAD family member 1; SMAD mothers against DPP homolog 1 Drosophila; Spinal muscular atrophy distal with upper limb predominance; TGF beta signaling protein 1; Transforming growth factor beta signaling protein 1; SMAD1_HUMAN; MAD homolog 1; Mothers against DPP homolog 1; Mad-related protein 1; SMAD family member 1; Smad1; Transforming growth factor-beta-signaling protein 1.

Background information

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq].