Antigen affinity purified
Polyclonal (rabbit origin)
Western blot: 0.1-0.5ug/ml
Rabbit (Oryctolagus cuniculus)
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Optimal dilution of the SMAD4 antibody should be determined by the researcher.
This SMAD4 antibodyis to be used only for research purposes and not for diagnostics..
Amino acids EIHLHRALQLLDEVLHTMPIADPQPLD of human SMAD4 were used as the immunogen for the SMAD4 antibody.
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
After reconstitution, the SMAD4 antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
SMAD4 (Mothers Against Decapentaplegic Drosophila Homolog of 4), also known as MADH4 or DPC4, is a protein that in humans is encoded by the SMAD4 gene. It belongs to the Darfwin family of proteins that modulate members of the TGFb protein superfamily. Hahn et al. (1996) identified the SMAD4 gene on chromosome 18q21.1. Howe et al. (1998) identified the SMAD4 gene within a region on 18q21.1 defined by linkage analysis in kindred with juvenile polyposis syndrome. To test directly the hypothesis that the SMAD4 gene is a tumor suppressor that is critical for transmitting signals from transforming growth factor-beta and related ligands. SMAD4 plays a pivotal role in signal transduction of the transforming growth factor beta superfamily cytokines by mediating transcriptional activation of target genes.