Antigen affinity purified
Polyclonal (rabbit origin)
Rabbit (Oryctolagus cuniculus)
Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
0.5mg/ml if reconstituted with 0.2ml sterile DI water
This SMAD4 antibodyis to be used only for research purposes and not for diagnostics..
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
An amino acid sequence from the N-terminus of human SMAD4 (RLWRWPDLHKNELKHVK) was used as the immunogen for this SMAD4 antibody (100% homologous in human, mouse and rat).
The stated application concentrations are suggested starting amounts. Titration of the SMAD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
After reconstitution, the Smad4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
Mothers Against Decapentaplegic Drosophila Homolog of 4, also known as MADH4 or DPC4, is a protein that in humans is encoded by the SMAD4 gene. It belongs to the Darfwin family of proteins that modulate members of the TGFbeta protein superfamily. Hahn et al.(1996) identified the gene on chromosome 18q21.1. Howe et al.(1998) identified the gene within a region on 18q21.1 defined by linkage analysis in a kindred with juvenile polyposis syndrome. To test directly the hypothesis that the SMAD4 gene is a tumor suppressor that is critical for transmitting signals from transforming growth factor-beta and related ligands. SMAD4 plays a pivotal role in signal transduction of the transforming growth factor beta superfamily cytokines by mediating transcriptional activation of target genes.