SMAD2/3 Antibody

Basic information

Name

SMAD2/3 Antibody

size

0.1mg

Catalog number

R30257

Price

389 €

Details & buy    

Extended information

Tested applications

WB

Uniprot #

Q15796

Recognised antigen

SMAD2/3

Category

Antibody

French translation

anticorps

Conjugation

Unconjugated

Purity

Antigen affinity

Clone

Polyclonal antibody

Recommended dilutions

Western blot: 0.5-1ug/ml

Form

Antigen affinity purified

Clonality

Polyclonal (rabbit origin)

Host animal

Rabbit (Oryctolagus cuniculus)

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Intented use

This SMAD2/3 antibodyis to be used only for research purposes and not for diagnostics..

Properties

If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Immunogen

An amino acid sequence from the C-terminus of human SMAD2/3 (QSPNCNQRYGWHPATVC) was used as the immunogen for this SMAD2/3 antibody (100% homologous in human, mouse and rat).

Notes

The stated application concentrations are suggested starting amounts. Titration of the SMAD2/3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Species reactivity

Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.

Storage

After reconstitution, the SMAD2/3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.

Description

SMAD proteins transmit signals from transmembrane serine/threonine kinase receptors to the nucleus. Transforming growth factor (TGF)-beta stimulation leads to phosphorylation and activation of Smad2 and Smad3, which form complexes with Smad4 that accumulate in the nucleus and regulate transcription of target genes. Smad2 and Smad3 share highly homology. SMAD2/3 signal transduction appeared to be important in the regulation of muscle-specific genes. SMAD proteins transmit signals from transmembrane serine/threonine kinase receptors to the nucleus. Smad2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development. Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development.