IHC, IF, WB, ELISA
Antigen affinity purified
Polyclonal (rabbit origin)
Rabbit (Oryctolagus cuniculus)
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunofluorescence: 1:25,IHC (Paraffin): 1:25,Western blot: 1:1000
This SMAD1 antibodyis to be used only for research purposes and not for diagnostics..
Titration of the SMAD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
This SMAD1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 163-196 amino acids from the Central region of human SMAD1.
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
Aliquot the SMAD1 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
SMADs are intracellular proteins that transduce extracellular signals from transforming growth factor beta ligands to the nucleus where they activate downstream gene transcription. The SMADs, which form a trimer of two receptor-regulated SMADs and one co-SMAD, act as transcription factors that regulate the expression of certain genes. There are three classes of SMADs, SMAD1 belongs to the receptor-regulated class (R-SMAD) which includes SMAD2, SMAD3, SMAD5 and SMAD8/9. [Wiki]