SMAD2 Antibody

Basic information

Name

SMAD2 Antibody

size

0.08 ml

Catalog number

F50346-0.08ML

Price

199 €

Details & buy    

Extended information

Recognised antigen

SMAD2

Uniprot #

Q15796

Category

Antibody

French translation

anticorps

Conjugation

Unconjugated

Tested applications

IF, WB, ELISA

Purity

Antigen affinity

Clone

Polyclonal antibody

Form

Antigen affinity purified

Clonality

Polyclonal (rabbit origin)

Host animal

Rabbit (Oryctolagus cuniculus)

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Recommended dilutions

Immunofluorescence: 1:200,Western blot: 1:1000

Intented use

This SMAD2 antibodyis to be used only for research purposes and not for diagnostics..

Immunogen

A portion of amino acids 201-230 from the human protein was used as the immunogen for this SMAD2 antibody.

Notes

Titration of the SMAD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Properties

If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Species reactivity

Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.

Storage

Aliquot the SMAD2 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.

Description

The protein belongs to the SMAD, a family of proteins similar to the proteins of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.