Smad3 (phospho-S423/S425) Rabbit monoclonal antibody
100ul
BS9938M-100ul
548 €
P84022
~ 55 kDa
anticorps
Human, Mouse
WB ICC/IF IHC
Oryctolagus cuniculus
Recombinant antibody.
Protein A affinity purified
WB: 1:1000 ICC/IF: 1:50-1:200 IHC: 1:50-1:200
For research use only, not for use in diagnostic procedure.
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
This antibody detects endogenous levels of Smad3 only when phosphorylated at Ser423 and/or Ser425.
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Western blot (WB) analysis of Smad3 (phospho-S423/S425) Rabbit mAb at 1:1000 dilution Lane1:A431 whole cell lysate Lane2:A431 whole cell lysate treated with TGF β
Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by Bioworld. Rabbit antibodies are very stable and can be stored for several days at room temperature. Bioworld adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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