Smad4 polyclonal antibody

Basic information

Name

Smad4 polyclonal antibody

size

100ul

Catalog number

BS61802-100ul

Price

425 €

Extended information

Applications

WB

Swiss-Prot

Q13485

BiowMW

~ 70 kDa

French translation

anticorps

Reactivity

Human,Mouse,Rat

Application_all

WB: 1:500~1:1000

Group

Polyclonals and antibodies

Immunogen

Synthetic peptide, corresponding Human SMAD4.

Specificity

SMAD4 pAb detects endogenous levels of SMAD4 protein.

Note

For research use only, not for use in diagnostic procedure.

Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Purification&Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Alternative Name

Mothers against decapentaplegic homolog 4; MAD homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4; SMAD 4; Smad4; hSMAD4; SMAD4; DPC4, MADH4

Usage

Western blot (WB) analysis of SMAD4 pAb at 1:500 dilution Lane1:A2780 whole cell lysate(40ug) Lane2:H1792 whole cell lysate(40ug) Lane3:The Spleen tissue lysate of Mouse(40ug) Lane4:The Spleen tissue lysate of Rat(40ug)

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.