Smad2/3 (phospho-T8) polyclonal antibody
100ul
AP0326-100ul
448 €
WB
anticorps
~ 55,60 kDa
P84022/Q15796
Human,Mouse,Rat
WB: 1:1000~1:2000
Polyclonals and antibodies
For research use only, not for use in diagnostic procedure.
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Synthetic phosphopeptide derived from human Smad2/3 around the phosphorylation site of Threonine 8.
p-Smad2/3 (T8) polyclonal antibody detects endogenous levels of Smad2/3 protein only when phosphorylated at Thr8.
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Western blot (WB) analysis of Smad2/3 (phospho-T8) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:HEK293T treated with TNFα(20ng/ml)for 10 min whole cell lysate Lane3:The Heart tissue lysate of Mouse
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2; MADR2
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