Smad2/3 (phospho-T8) polyclonal antibody

Basic information

Name

Smad2/3 (phospho-T8) polyclonal antibody

size

50ul

Catalog number

BS4590-50ul

Price

318 €

Details & buy    

Extended information

Applications

WB

French translation

anticorps

Reactivity

Human,Mouse

BiowMW

~ 55, 60 kDa

Swiss-Prot

P84022/Q15796

Application_all

WB: 1:500~1:1000

Group

Polyclonals and antibodies

Note

For research use only, not for use in diagnostic procedure.

Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Immunogen

Synthetic phosphopeptide derived from human Smad2/3 around the phosphorylation site of Threonine 8.

Specificity

p-Smad2/3 (T8) polyclonal antibody detects endogenous levels of Smad2/3 protein only when phosphorylated at Thr8.

Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Purification&Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Usage

Western blot (WB) analysis of p-Smad2/3 (T8) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40µg) Lane2:Hela whole cell lysate(40µg) Lane3:The Lung tissue lysate of Mouse(40µg) Lane4:SGC7901 whole cell lysate(40µg) Lane5:H9C2 whole cell lysate(40µg)

Alternative Name

Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2; MADR2