Smad2/3 (phospho-T8) polyclonal antibody

Basic information

Name

Smad2/3 (phospho-T8) polyclonal antibody

size

50ul

Catalog number

AP0326-50ul

Price

318 €

Extended information

Applications

WB

French translation

anticorps

BiowMW

~ 55,60 kDa

Swiss-Prot

P84022/Q15796

Reactivity

Human,Mouse,Rat

Application_all

WB: 1:1000~1:2000

Group

Polyclonals and antibodies

Note

For research use only, not for use in diagnostic procedure.

Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Immunogen

Synthetic phosphopeptide derived from human Smad2/3 around the phosphorylation site of Threonine 8.

Specificity

p-Smad2/3 (T8) polyclonal antibody detects endogenous levels of Smad2/3 protein only when phosphorylated at Thr8.

Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Purification&Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Usage

Western blot (WB) analysis of Smad2/3 (phospho-T8) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:HEK293T treated with TNFα(20ng/ml)for 10 min whole cell lysate Lane3:The Heart tissue lysate of Mouse

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Alternative Name

Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2; MADR2