Smad3 (Ser423 + Ser425) Antibody, FITC Conjugated

Basic information

Name

Smad3 (Ser423 + Ser425) Antibody, FITC Conjugated

size

0.1ml

Catalog number

bs-3425R-FITC

Price

350 €

Extended information

Gene ID number

4088

Conjugation

FITC

Swiss Prot

P84022

Tested applications

IF(IHC-P)

French translation

anticorps

Clonality

Polyclonal

Immunogen_range

380-425/425

Excitation Emission

494nm/518nm

Concentration

1ug per 1ul

Modification

Phosphorylation

Modification site

Ser423 + Ser425

Crossreactivity

Human, Mouse, Rat

Additional conjugation

Anti-FITC Antibody

Subcellular location

Cytoplasm, Nucleus

Recommended dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal antibody

Target Antigen

Smad3 Ser423 + Ser425

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Purification method

This antibody was purified via Protein A.

Long name

Smad3 (Ser423 + Ser425) Antibody, FITC Conjugated

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425 [CS(p-S)V(p-S)]

Specificity

This is a highly specific antibody against Smad3 Ser423 + Ser425. These phosphorylation sites are homologous across the species listed.

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Synonyms

LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Antigen background

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.