Smad3 (Ser213) Antibody, Cy5 Conjugated

Basic information

Name

Smad3 (Ser213) Antibody, Cy5 Conjugated

size

0.1ml

Catalog number

bs-5459R-Cy5

Price

368 €

Details & buy    

Extended information

Conjugation

Cy5

Gene ID number

4088

Additional conjugation

Cy5.5

Modification site

Ser213

Tested applications

IF(IHC-P)

French translation

anticorps

Clonality

Polyclonal

Concentration

1ug per 1ul

Target Antigen

Smad3 Ser213

Excitation Emission

625,650nm/670nm

Modification

Phosphorylation

Crossreactivity

Human, Mouse, Rat

Clone

Polyclonal antibody

Recommended dilutions

IF(IHC-P)(1:50-200)

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Purification method

This antibody was purified via Protein A.

Long name

Smad3 (Ser213) Polyclonal Antibody, Cy5 Conjugated

Specificity

This is a highly specific antibody against Smad3 Ser213.

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser213 [PM(p-S)PA]

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.

Synonyms

SMAD3phospho S213; SMAD3phospho Ser213; p-SMAD3Ser213; hMAD 3; hSMAD3; HSPC193; JV15 2; JV152; MAD mothers against decapentaplegic Drosophila homolog 3; MAD3; MADH 3; MADH3; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA and MAD related protein 3; SMAD 3; SMAD; SMAD-3; SMAD3_HUMAN.

Antigen background

Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling.