Smad2/3 Antibody, Cy3 Conjugated

Basic information

Name

Smad2/3 Antibody, Cy3 Conjugated

size

0.1ml

Catalog number

bs-3484R-Cy3

Price

350 €

Details & buy    

Extended information

Conjugation

Cy3

Modification site

None

Swiss Prot

Q15796

Target Antigen

Smad2/3

Gene ID number

40874088

Immunogen_range

30-80/467

Tested applications

IF(IHC-P)

French translation

anticorps

Modification

Unmodified

Clonality

Polyclonal

Concentration

1ug per 1ul

Crossreactivity

Human, Mouse, Rat

Subcellular location

Cytoplasm, Nucleus

Recommended dilutions

IF(IHC-P)(1:50-200)

Additional conjugation

cy3 conjugation kit

Excitation Emission

512,550nm/570,615nm

Clone

Polyclonal antibody

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Purification method

This antibody was purified via Protein A.

Long name

Smad2/3 Polyclonal Antibody, Cy3 Conjugated

Specificity

This is a highly specific antibody against Smad2/3.

Source

KLH conjugated synthetic peptide derived from human Smad2

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Synonyms

JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; Mad-related protein 2; SMAD family member 2; SMAD 2; Smad2

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-Smad2/3 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.

Antigen background

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.