Smad3 (Ser213) Antibody, Biotin Conjugated

Basic information

Name

Smad3 (Ser213) Antibody, Biotin Conjugated

size

0.1ml

Catalog number

bs-5459R-Biotin

Price

350 €

Details & buy    

Extended information

Excitation Emission

N/A

Gene ID number

4088

Conjugation

Biotin

Modification site

Ser213

Tested applications

WB, IHC-P

French translation

anticorps

Clonality

Polyclonal

Concentration

1ug per 1ul

Additional conjugation

Biotinylated

Target Antigen

Smad3 Ser213

Modification

Phosphorylation

Crossreactivity

Human, Mouse, Rat

Clone

Polyclonal antibody

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Recommended dilutions

WB(1:100-1000), IHC-P(1:100-500)

Purification method

This antibody was purified via Protein A.

Long name

Smad3 (Ser213) Polyclonal Antibody, Biotin Conjugated

Specificity

This is a highly specific antibody against Smad3 Ser213.

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser213 [PM(p-S)PA]

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Synonyms

SMAD3phospho S213; SMAD3phospho Ser213; p-SMAD3Ser213; hMAD 3; hSMAD3; HSPC193; JV15 2; JV152; MAD mothers against decapentaplegic Drosophila homolog 3; MAD3; MADH 3; MADH3; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA and MAD related protein 3; SMAD 3; SMAD; SMAD-3; SMAD3_HUMAN.

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Antigen background

Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling.