Vial with antibody
Human, Mouse, Rat
SMAD family member 3
, Immunohistochemistry: 1:50~1:100
Store antibody at -20 grades C for up to one year.
This antibody detects endogenous level of Smad3 only when phosphorylated at serine 425.
LDS3, LDS1C, MADH3, JV15-2, HSPC193, HsT17436, PTP2C, SHPTP2, MAD-3, Mad3, Mothers against DPP homolog 3
Suspention, pH, azide
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Smad3 (Phospho-Ser425) antibody was made against a peptide sequence around phosphorylation site of serine 425 (C-S-S-V-S (p) ) derived from Human Smad3.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.