anti-SMAD3 Antibody

Basic information

Name

anti-SMAD3 Antibody

size

0.05 mg

Catalog number

49-540

Price

180 €

Details & buy    

Extended information

NCBI GENE nr

4088

GENE symbol

SMAD3

Protein nr

P84022

Ncbi nr

P84022

Ab raised in

Rabbit

Omim nr

51338669

French translation

anticorps

Type

Polyclonal

Vial with antibody

in solution

Spicific to

pSer423/425

Source

Homo sapiens

Antibodie's label

unlabelled serum

Antigene name

SMAD family member 3

Purity

Immunoaffinity Chromatography

Works with

Bovine, Chicken, Xenopus, Human, Mouse, Pig, Rat, Zebrafish

Immuno application

ELISA, Western Blot, immuno-histo-chemistry, immuno fluorescence

Antigen

SMAD3 antibody was made against amino acids 417 - 425 of SMAD3 (Human).

Suspention, pH, azide

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.

Keep at

Store SMAD3 antibody at 4 grades C or -20 grades C. As with all antibodies avoid freeze/thaw cycles.

Antibody advice

SMAD3 antibody can be used in Western Blot starting at 5 - 1 ug/mL, immunohistochemistry starting at 10 ug/mL, and flow cytometry.

Properties

If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Gene names

SMAD3, HSPC193, HMAD-3, HsT17436, LDS1C, LDS3, Mad homolog JV15-2, MAD3, MADH3, Mothers against DPP homolog 3, JV15-2, SMA- and MAD-related protein 3, SMAD family member 3, MAD homolog 3, HSMAD3, Mad protein homolog, SMAD 3

Gene info

Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-β (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily. Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway. Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response.