Vial with antibody
SMAD family member 3
Western Blot, immuno-histo-chemistry
Suspention, pH, azide
PBS, 0.2% gelatin, 0.05% sodium azide.
Bovine, Dog, Human, Macaque, Monkey, Mouse, Pig, Rat
SMAD3 antibody was made against a portion of amino acids 100 - 150 of SMAD3 (Human).
SMAD3 antibody can be used in ELISA, Western Blot, and immunohistochemistry starting at 5 ug/mL.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
SMAD3 antibody can be stored short term 4 grades C. For long term storage aliquot and store at -20 grades C. As with all antibodies avoid freeze/thaw cycles.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
SMAD3, HSPC193, HMAD-3, HsT17436, LDS1C, LDS3, Mad homolog JV15-2, MAD3, MADH3, Mothers against DPP homolog 3, JV15-2, SMA- and MAD-related protein 3, SMAD family member 3, MAD homolog 3, HSMAD3, Mad protein homolog, SMAD 3
SMAD3, a member of SMAD family of proteins, is involved in TGF-β signaling cascade. A human homolog of Drosophila Mad protein, SMAD3 consists of N-terminal DNA-binding MH1 domain, a linker region and a C-terminal MH2 domain.It is a receptor regulated SMAD (R-SMAD) that functions downstream of TGF-β and activin receptors and mediates their signaling. It is recruited by SARA (Smad anchor for receptor activation) to the receptor kinase for phosphorylation. Upon phosphorylation, SMAD3 disassociates from SARA, forms a complex with SMAD4 and transmigrates into the nucleus where it complexes with other cofactors and acts as a transcription factor. It has an indispensable role in cell proliferation, differentiation, apoptosis and formation of ECM. Loss of SMAD3 results in Childhood T-cell leukemia. Smad 3 (Mothers against decapentaplegic homolog 3), a transcriptional regulator, is activated by TGF-β and Activin 1 receptor kinase. SMAD3 recognizes an 8 bp palindromic sequence GTCTAGAC. Upon phosphorylation at two extreme C-terminal serines, SMAD3 is translocated into the nucleus where is forms complexes other SMAD proteins, depending on the activation pathway, and regulates the transcription of target genes.