Quantity per ml
Vial with antibody
ELISA, Western Blot
SMAD family member 4
Aliquot and store at -20 grades C. Minimize freezing and thawing.
SMAD4 antibody was made against a 12 amino acid synthetic peptide near the C-Terminus of SMAD4.
Suspention, pH, azide
SMAD4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
SMAD4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 60-65 kDa band seen in A431 cell lysate. Recommended for use at 0.5-2 ug/mL.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
MADH4, JIP, DPC4, SMAD4, MAD, mothers against decapentaplegic homolog 4 (Drosophila), Mothers against decapentaplegic, Drosophila, homolog of, 4, MAD (mothers against decapentaplegic, Drosophila) homolog 4, MADH4