anti-SMAD7 Antibody

Basic information

Name

anti-SMAD7 Antibody

size

50 ug

Catalog number

27-477

Price

180 €

Details & buy    

Extended information

NCBI GENE nr

4092

Works with

Human

GENE symbol

SMAD7

Ab raised in

Rabbit

Protein nr

O15105

KDa

46 kDa

Omim nr

5174517

Quantity per ml

1 mg/ml

Ncbi nr

NP_005895

French translation

anticorps

Type

Polyclonal

Vial with antibody

Lyophilized

Source

Homo sapiens

Antibodie's label

unlabelled serum

Immuno application

ELISA, Western Blot

Antigene name

SMAD family member 7

Gene names

SMAD7, CRCS3, MADH7, MADH8

Purity

Antibody is purified by peptide affinity chromatography method.

Antigen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD7.

Suspention, pH, azide

Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.

Properties

If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Keep at

For short periods of storage (days) store at 4 grades C. For longer periods of storage, store SMAD7 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.

Antibody advice

SMAD7 antibody can be used for detection of SMAD7 by ELISA at 1:12500. SMAD7 antibody can be used for detection of SMAD7 by western blot at 1.0 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Gene info

Smad7 acts to functionally inactivate RB and de-repress E2F without blocking the activation of TbetaRI and the nuclear translocation of Smad2/3, allowing TGF-beta1 to exert effects in a cancer cell that is resistant to TGF-beta1-mediated growth inhibition.