anti-SMAD5 Antibody

Basic information

Name

anti-SMAD5 Antibody

size

100 ug

Catalog number

27-476

Price

180 €

Details & buy    

Extended information

NCBI GENE nr

4090

GENE symbol

SMAD5

Ab raised in

Rabbit

Protein nr

Q99717

Quantity per ml

1 mg/ml

Omim nr

47778931

French translation

anticorps

Type

Polyclonal

Vial with antibody

Lyophilized

Source

Homo sapiens

Ncbi nr

NP_001001420

Antibodie's label

unlabelled serum

Immuno application

ELISA, Western Blot

Antigene name

SMAD family member 5

KDa

51 kDa, 52 kDa, 52 kDa

Works with

Human, Mouse, Rat, Dog

Gene names

SMAD5, DWFC, JV5-1, MADH5

Purity

Antibody is purified by protein A chromatography method.

Antigen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD5.

Suspention, pH, azide

Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μL of distilled water. Final antibody concentration is 1 mg/mL.

Properties

If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Keep at

For short periods of storage (days) store at 4 grades C. For longer periods of storage, store SMAD5 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.

Antibody advice

SMAD5 antibody can be used for detection of SMAD5 by ELISA at 1:62500. SMAD5 antibody can be used for detection of SMAD5 by western blot at 2.5 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Gene info

SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection.