Vial with antibody
ELISA, Western Blot
SMAD family member 5
Human, Mouse, Rat, Dog
SMAD5, DWFC, JV5-1, MADH5
Antibody is purified by peptide affinity chromatography method.
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD5.
Suspention, pH, azide
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
For short periods of storage (days) store at 4 grades C. For longer periods of storage, store SMAD5 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
SMAD5 antibody can be used for detection of SMAD5 by ELISA at 1:62500. SMAD5 antibody can be used for detection of SMAD5 by western blot at 1.0 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection.