anti-SMAD4 Antibody

Basic information

Name

anti-SMAD4 Antibody

size

100 ug

Catalog number

27-474

Price

180 €

Extended information

NCBI GENE nr

4089

Works with

Human

GENE symbol

SMAD4

Ab raised in

Rabbit

Protein nr

Q13485

KDa

60 kDa

Omim nr

4885457

Quantity per ml

1 mg/ml

Ncbi nr

NP_005350

French translation

anticorps

Type

Polyclonal

Vial with antibody

Lyophilized

Source

Homo sapiens

Antibodie's label

unlabelled serum

Immuno application

ELISA, Western Blot

Antigene name

SMAD family member 4

Gene names

SMAD4, JIP, DPC4, MADH4, MYHRS

Purity

Antibody is purified by protein A chromatography method.

Antigen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD4.

Suspention, pH, azide

Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μL of distilled water. Final antibody concentration is 1 mg/mL.

Properties

If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Keep at

For short periods of storage (days) store at 4 grades C. For longer periods of storage, store SMAD4 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.

Antibody advice

SMAD4 antibody can be used for detection of SMAD4 by ELISA at 1:312500. SMAD4 antibody can be used for detection of SMAD4 by western blot at 1.25 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Gene info

SMAD4 is one of the Smad family members, which are essential intracellular signalling components of the transforming growth factor-beta (TGF-beta) superfamily. Smad2 and Smad3 are structurally highly similar and mediate TGF-beta signals. Smad4 is distantly related to Smads 2 and 3, and forms a heteromeric complex with Smad2 after TGF-beta or activin stimulation. TGF-beta induces heteromeric complexes of Smads 2, 3 and 4, and their concomitant translocation to the nucleus, which is required for efficient TGF-beta signal transduction