Vial with antibody
Human, Mouse, Rat
Predicted: 80, 83 kDa Observed: 78 kDa
ELISA, Western Blot, immuno-histo-chemistry
SMAD specific E3 ubiquitin protein ligase 1
Suspention, pH, azide
SMURF1 antibody is supplied in PBS containing 0.02% sodium azide.
SMURF1 antibody is affinity chromatography purified via peptide column.
SMURF1 antibody was made against a 16 amino acid peptide near the amino terminus of human SMURF1.
SMURF1 antibody can be stored at 4 grades C for three months and -20 grades C, stable for up to one year.
SMAD specific E3 ubiquitin protein ligase 1, SMAD ubiquitination regulatory factor 1, E3 ubiquitin-protein ligase SMURF1
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
SMURF1 body can be used for detection of SMURF1 by Western blot at 1 - 2 ug/ml. Antibody can also be used for Immunohistochemistry starting at 5 ug/mL.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
SMURF1 antibody is human, mouse and rat reactive. At least three isoforms of SMURF1 are known to exist this antibody will recognize all three isoforms. SMURF1 is predicted to not cross-react with SMURF2.
SMURF1 (SMAD ubiquitin regulatory factor 1) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF1 negatively regulates osteoblast activity and response to bone morphogenesis protein (BMP) through controlling MEKK2 degradation (3). SMURF1 has recently been shown to play a role in breast cancer cell migration and invasion through the downregulation of RhoA (4).