Vial with antibody
Human, Mouse, Rat
Predicted: 82 kDa Observed: 85 kDa
SMAD specific E3 ubiquitin protein ligase 2
ELISA, Western Blot, immuno-histo-chemistry, immuno fluorescence
Suspention, pH, azide
SMURF2 Antibody is supplied in PBS containing 0.02% sodium azide.
SMURF2 antibody is affinity chromatography purified via peptide column.
SMURF2 antibody was made against a 17 amino acid peptide near the amino terminus of human SMURF2.
SMURF2 antibody is human, mouse and rat reactive. SMURF2 is predicted to not cross-react with SMURF1.
SMURF2 Antibody: E3 ubiquitin-protein ligase SMURF2, SMAD ubiquitination regulatory factor 2, hSMURF2
SMURF2 antibody can be stored at 4 grades C for three months and -20 grades C, stable for up to one year.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
SMURF2 antibody can be used for detection of SMURF2 by Western blot at 1 - 2 ug/ml. Antibody can also be used for Immunohistochemistry at 5 ug/mL. For Immunoflorescence start at 20 ug/mL.
SMURF2 (SMAD ubiquitin regulatory factor 2) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF2 is widely expressed and was initially identified as an inhibitor of TGF-beta/BMP signaling by targeting R-Smads and TGF type I receptor for ubiquitination and degradation (3). Studies have shown that SMURF2 functions as a tumor suppressor by maintaining genomic stability through targeting RNF20 (3). SMURF2 associates constitutively with SMAD7 (4).