Mouse Monoclonal Anti-SMAD5 Antibody (4B10-B10-B6)

Basic information

Name

Mouse Monoclonal Anti-SMAD5 Antibody (4B10-B10-B6)

size

100 µg

Catalog number

A1339-100

Price

338 €

Details & buy    

Extended information

Gene number

4090

Reacts with

Human

Raised in

Mouse

Protein number

Q99717

French translation

anticorps

Latin name

Mus musculus

Physical state

Colorless liquid

Purity

Affinity purified

Research area

Signal Transduction

Delivery

Shipped to your lab under gel pack

Clonality

Monoclonal Antibody (Clone#4B10-B10-B6)

Description

Mouse Monoclonal antibody to detect SMAD5

Released

This antibody is available to order since May 2017

Precautions

Manifactured in USA. For Research Use Only! Not to be used in humans

Approved applications with antibody dilution

Western blot; 1:1000, Immunofluorescence; 1:75, Flow cytometryM; 1:100

Immunogen

Recombinant human SMAD5 (C terminal) protein fragments expressed in E.coli

Antibody's positive controles

Western blot: Hela, Jurkat and K562 cell lysates; Immunofluorescence: HeLa cells; Flow cytometry: Jurkat cells

Handling and storage

Before use gently mix the antibody solution. Store this antibody at -20 °C for 12 months. Refer to the label on vial

Properties

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Scientific context

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

Target

SMAD5 (Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5, SMAD 5, Smad5, hSmad5)

Package form

Affinity purified antibody is supplied in liquid form in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol

Additional description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.