Target of this antibody:
Concentration of antibody:
Clonality of antibody:
This antibody has been succesfully tested for use in following methods:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide from human Smad 7
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
Alternative names of antibody target:
SMAD7 , MADH7, MADH8 , Smad7; hSMAD7; SMAD family member 7
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-Smad7 polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Other applications have not been determined. The antibody detects ~46 kDa Smad-7 from samples of human, mouse, and rat origins. Reactivity to other species has not been determined.
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1, Smad5, and Smad8 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF-β and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities throμgh interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.