anti-Smad5 Antibody

Basic information

Name

anti-Smad5 Antibody

size

Smad5 Antibody

Catalog number

3465-100

Price

338 €

Details & buy    

Extended information

Target of this antibody:

Smad5

Antibody host:

Rabbit

Form of antibody:

Liquid

Shipping conditions:

gel pack

Concentration of antibody:

0.5 mg/ml

French translation

anticorps

Isotype:

Rabbit IgG

Clonality of antibody:

Polyclonal

This antibody has been succesfully tested for use in following methods:

Western blot

Purification method:

Affinity purified

This antibody reacts with:

Human, mouse, rat

Storage information:

Shelf time: 12 months in temp.: -20 deg.C

How to handle this antibody:

The antibody solution should be gently mixed before use.

Alternative names of antibody target:

DKFZp781C1895, DKFZp781O1323, DwFlow cytometry, JV5-1, MADH5

Immunogene sequence:

Synthetic peptide corresponding to amino acids residues around 252 of human Smad5

Properties

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Formulation of this antibody:

100 µg (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Recommended usage:

Western blotting (1-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~60 kDa Smad5 of human, mouse, and rat origins. Reactivity to other species has not been tested.

Background information:

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1, Smad5, and Smad8 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF-β and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities throμgh interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.