Rat SMURF2(Smad Specific E3 Ubiquitin Protein Ligase 2) ELISA Kit

Basic information

Name

Rat SMURF2(Smad Specific E3 Ubiquitin Protein Ligase 2) ELISA Kit

size

96T

Catalog number

ELK8152

Price

608 €

Details & buy    

Extended information

Assay length

3h

Standard

50ng/mL

Assay Type

Sandwich

Sensitivity

0.27ng/mL

Detection range

0.781-50ng/mL

Research Area

Enzyme & Kinase;

Latin name

Rattus norvegicus

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Alternative Names

SMAD ubiquitination regulatory factor 2; E3 ubiquitin-protein ligase SMURF2

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Smad Specific E3 Ubiquitin Protein Ligase 2 (SMURF2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Smad Specific E3 Ubiquitin Protein Ligase 2 (SMURF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Smad Specific E3 Ubiquitin Protein Ligase 2 (SMURF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Smad Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.