Rabbit Smad3(Mothers Against Decapentaplegic Homolog 3) ELISA Kit

Basic information

Name

Rabbit Smad3(Mothers Against Decapentaplegic Homolog 3) ELISA Kit

size

96T

Catalog number

ELK7805

Price

738 €

Extended information

Assay length

3h

Standard

10ng/mL

Assay Type

Sandwich

Sensitivity

0.056ng/mL

Detection range

0.156-10ng/mL

Alternative Names

JV15-2; MADH3

Latin name

Oryctolagus cuniculus

Research Area

Signal transduction;Tumor immunity;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

About

Rabbits are used for polyclonal antibody production by ELK Biotech. Rabbit antibodies are very stable and can be stored for several days at room temperature. ELK Biotech adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mothers Against Decapentaplegic Homolog 3 (Smad3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mothers Against Decapentaplegic Homolog 3 (Smad3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mothers Against Decapentaplegic Homolog 3 (Smad3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mothers Against Decapentaplegic Homolog 3 (Smad3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.