Human Smad3/MADH3 (Mothers Against Decapentaplegic Homolog 3) CLIA Kit

Basic information

Name

Human Smad3/MADH3 (Mothers Against Decapentaplegic Homolog 3) CLIA Kit

size

96Test

Catalog number

E-CL-H0868-96T

Price

541 €

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Extended information

UNIProt ID

NA

Target Synonym

NA

Target Species

Human

Type

Sandwich

Sensitivity

0.09ng/mL

Target Name

Smad3/MADH3

Detection Type

Colormetric

Detection Range

0.16~10ng/mL

Tested Sample Types

Serum, Plasma, Cell supernatant

Product Name

Human Smad3/MADH3 (Mothers Against Decapentaplegic Homolog 3) CLIA Kit

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Test principle

This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Smad3/MADH3 . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Smad3/MADH3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Smad3/MADH3 , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Smad3/MADH3 . You can calculate the concentration of Human Smad3/MADH3 in the samples by comparing the RLU value of the samples to the standard curve.