Rabbit Anti Human,Mouse SMAD2 Antibody-Non-conjugated

Basic information

Name

Rabbit Anti Human,Mouse SMAD2 Antibody-Non-conjugated

size

50ug

Catalog number

CSB-PA15557A0Rb_50ug

Price

212 €

Extended information

Antibody isotype

IgG

Purity

>95%

Immunogen species

Human

UniProt ID

Q15796

French translation

anticorps

Species reactivity

Human,Mouse

Conjugation

Non-conjugated

Purification method

Protein G purified

Clonality

Polyclonal antibody

Antibody category

Polyclonal Antibodies

Format

Liquid, diluted in buffer

Synonym name

SMAD2 Human, Mouse Antibody

Latin name

Mus musculus,Oryctolagus cuniculus

Short name of the antigen

Mothers against decapentaplegic homolog 2

Long name

Rabbit Antibody against Human, Mouse SMAD2, Non-conjugated

Immunogen type

Recombinant human Mothers against decapentaplegic homolog 2 protein (2-467AA)

Buffer composition

Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Tested applications & recommended dilutions

ELISA,WB,IHC,IF;Recommended dilution:WB:1:2000-1:5000,IHC:1:20-1:200,IF:1:50-1:200

Alias

Mothers against decapentaplegic homolog 2MAD homolog 2Mothers against DPP homolog 2,JV18-1,Mad-related protein 2,hMAD-2,SMAD family member 2,SMAD 2,Smad2,hSMAD2,SMAD2,MADH2,MADR2

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.

Shipping and storage

SMAD2 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.

About

Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Properties

If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Background

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.