Area of research
Cytokines & Growth Factors
Polyclonal Antibodies, Purified
IHC: 1:50-1:100, WB:1:500-1:1000
Method of Purification
Smad3 antibody was purified by affinity chromatography
Store at -20 deg C in small aliquots. Avoid repeat freeze/thaw cycles
Form & Buffer
Provided in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% NaN3 and 50% glycerol
Smad3 antibody (Ser425) detects endogenous levels of Smad3 only when phosphorylated at serine 425
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Type of Immunogen
Smad3 antibodies were raised in Rabbit using a synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of serine 425 (C-S-S-V-SP) ..