Area of research
Polyclonal Antibodies, Purified
Human: Ser425, Mouse: Ser425, Rat: Ser425
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:40000
Store at -20 deg C in small aliquots. Avoid repeated freeze-thaw cycles
SMAD3 antibody detects endogenous levels of Smad3 only when phosphorylated at serine 425
Form & Buffer
Purified IgG in PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% NaN3 and 50% glycerol
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Type of Immunogen
SMAD3 antibodies were raised in Rabbit using a synthesized phospho derived from human Smad3 around the phosphorylation site of serine 425 (C-S-S-V-SP) .