Area of research
DNA & RNA
Polyclonal Antibodies, Purified
WB: 1:500 - 1:1000, ELISA: 1:20000
Method of Purification
Smad3 antibody was purified by affinity chromatography
Aliquot and store at -20 deg C. Avoid repeated freeze thaw cycles
Smad3 antibody (Ser204) detects endogenous levels of Smad3 only when phosphorylated at serine 204
Mouse, Rat - Reactive with proteins phosphorylated as follows: Human: Ser204, Mouse: Ser204, Rat: Ser204
Form & Buffer
Provided in liquid format, PBS buffer, pH 7.4, 150 mM NaCl with added preservative 0.02% NaN3 and 50% glycerol
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Type of Immunogen
Smad3 antibodies were raised in Rabbit using a synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of serine 204 (A-G-SP-P-N) .