Area of research
Polyclonal Antibodies, Purified
Method of Purification
SMAD3 antibody was purified by affinity chromatography
Aliquot and store at -20 deg C. Avoid repeated freeze thaw cycles
ELISA: 1:20,000 - 1:80,000, WB: 1:500 - 1:1,000, IHC: 1:100 - 1:500
Type of Immunogen
SMAD3 antibodies were raised in Rabbit using a synthesized non-phosphopeptide derived from human SMAD3 .
Form & Buffer
Provided in liquid format, PBS buffer, pH 7.4, 150 mM NaCl with added preservative 0.02% NaN3 and 50% glycerol
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.