Area of research
DNA & RNA
Dot Blot; ICC; IP; WB; FC
Human, Mouse, Rat. Other species not studied
WB: 0.2-2 ug/ml, IP: 100-500 ug/sample, FC: 0.5-2 ug/sample, ICC: 2-100 ug/ml
Type of Immunogen
SMAD1 antibodies were raised in mouse using recombinant Human Smad Family Member 1 (Smad1)
Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Form & Buffer
SMAD1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% NaN3 (NaN3 ).
Method of Purification
SMAD1 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.